CRISPR-Cas9 Genome Editing
Programmable genome editing with Cas9 and guide RNAs, enabling targeted cuts, knockouts, repairs, and engineered sequence changes.
Core metadata
- ID: crispr_gene_editing
- Era: Modern
- First known date: 2013 (exact)
- Region: Global molecular biology research
- Review status: source_checked
- Maturity: established
Prerequisites
- Programmable Cas9 Nuclease (cas9_programmable_nuclease)
- NHEJ and HDR DNA Repair Editing (dna_repair_nhej_hdr)
- Genetic Engineering (genetic_engineering)
- PAM Recognition Constraint (pam_recognition_constraint)
- Single-Guide RNA Design (single_guide_rna_design)
Dependents
- Base Editing (base_editing)
- Bio-Enhancements (Genetic, Cybernetic Augmentation) (bio_enhancements_genetic_cybernetic_augmentation)
- CRISPR Delivery: AAV, LNP, and RNP (crispr_delivery_aav_lnp_rnp)
- CRISPR Off-Target Profiling (crispr_off_target_profiling)
- CRISPRi and CRISPRa (crispri_crispra)
- Epigenetic Engineering & Rejuvenation (epigenetic_engineering_rejuvenation)
- Ex Vivo CRISPR Cell Therapy (ex_vivo_crispr_cell_therapy)
- Engineered PAM Specificity Variants (pam_specificity_engineering)
- Personalized Medicine (personalized_medicine)
- Pooled CRISPR Screens (pooled_crispr_screens)
Fields
Field lanes
- Genome Editing / CRISPR-Cas: Editing Platforms
Node sources
- Multiplex Genome Engineering Using CRISPR/Cas Systems (Science / PubMed Central, 2013, primary_paper) • Supports: node, maturity
- RNA-guided human genome engineering via Cas9 (Science / PubMed, 2013, primary_paper) • Supports: node, maturity
- The Nobel Prize in Chemistry 2020 (Nobel Prize, 2020, museum) • Supports: node, maturity
Prerequisite edge evidence
Edge/source evidence summary:
- Prerequisite edges: 5
- Average edge confidence: 80%
- Prerequisite sources: 6
- expert_inference: 2
- primary_source: 2
- review: 1
| Prerequisite | Type | Confidence | Evidence level | Note | Sources |
|---|---|---|---|---|---|
| Genetic Engineering (genetic_engineering) | historical_predecessor | 76% | review | Genetic engineering is the broader predecessor field for genome-engineering methods, but it is not a discrete component required by the CRISPR-Cas9 editing mechanism. |
|
| Programmable Cas9 Nuclease (cas9_programmable_nuclease) | required | 94% | primary_source | Early CRISPR-Cas9 genome editing requires Cas9 nuclease activity as the programmable DNA-cutting component directed by guide RNA. |
|
| Single-Guide RNA Design (single_guide_rna_design) | enabling | 68% | expert_inference | Single-Guide RNA Design provides a capability that enables this technology without being the only possible path. |
|
| PAM Recognition Constraint (pam_recognition_constraint) | required | 93% | primary_source | CRISPR-Cas9 genome editing requires a target DNA sequence with a compatible PAM because Cas9 DNA recognition and cleavage strictly require PAM. |
|
| NHEJ and HDR DNA Repair Editing (dna_repair_nhej_hdr) | enabling | 68% | expert_inference | NHEJ and HDR DNA Repair Editing provides a capability that enables this technology without being the only possible path. |
|
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