Engineered PAM Specificity Variants
Engineering or directed evolution of Cas enzymes with altered protospacer-adjacent motif specificities to expand the set of targetable genome sites.
Core metadata
- ID: pam_specificity_engineering
- Era: Modern
- First known date: 2015 (exact)
- Region: Global molecular biology research
- Review status: source_checked
- Maturity: established
Prerequisites
- CRISPR-Cas9 Genome Editing (crispr_gene_editing)
- PAM Recognition Constraint (pam_recognition_constraint)
- Protein Engineering (protein_engineering)
Dependents
- None.
Fields
Field lanes
- Genome Editing / CRISPR-Cas: Editing Platforms
Node sources
- Engineered CRISPR-Cas9 nucleases with altered PAM specificities (Nature, 2015, primary_paper) • Supports: node, maturity
Prerequisite edge evidence
Edge/source evidence summary:
- Prerequisite edges: 3
- Average edge confidence: 85%
- Prerequisite sources: 3
- primary_source: 3
| Prerequisite | Type | Confidence | Evidence level | Note | Sources |
|---|---|---|---|---|---|
| CRISPR-Cas9 Genome Editing (crispr_gene_editing) | required | 78% | primary_source | Wild-type CRISPR-Cas9 genome editing exposed PAM targeting constraints that engineered PAM-specificity variants were designed to relax. |
|
| PAM Recognition Constraint (pam_recognition_constraint) | required | 90% | primary_source | Engineering altered PAM specificity depends on the Cas9 PAM-recognition constraint that defines which target sites wild-type Cas9 can recognize. |
|
| Protein Engineering (protein_engineering) | required | 86% | primary_source | Altered PAM-specificity variants are engineered Cas9 proteins produced through structural design, selection, and directed evolution. |
|
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