PAM Recognition Constraint
Requirement that Cas9 target DNA include a compatible protospacer-adjacent motif so Cas9 can recognize, unwind, and cleave the target sequence.
Core metadata
- ID: pam_recognition_constraint
- Era: Modern
- First known date: 2012 (exact)
- Region: United States, Europe, and global CRISPR research
- Review status: source_checked
- Maturity: established
Prerequisites
- Programmable Cas9 Nuclease (cas9_programmable_nuclease)
- Single-Guide RNA Design (single_guide_rna_design)
Dependents
- CRISPR-Cas9 Genome Editing (crispr_gene_editing)
- Engineered PAM Specificity Variants (pam_specificity_engineering)
Fields
Field lanes
- Genome Editing / CRISPR-Cas: Foundations
Node sources
- A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity (Science / PubMed, 2012, primary_paper) • Supports: node, maturity
- Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease (Nature, 2014, primary_paper) • Supports: node, maturity
Prerequisite edge evidence
Edge/source evidence summary:
- Prerequisite edges: 2
- Average edge confidence: 81%
- Prerequisite sources: 2
- primary_source: 2
| Prerequisite | Type | Confidence | Evidence level | Note | Sources |
|---|---|---|---|---|---|
| Programmable Cas9 Nuclease (cas9_programmable_nuclease) | required | 90% | primary_source | PAM recognition is a Cas9 target-DNA recognition constraint rather than a separate engineered variant. |
|
| Single-Guide RNA Design (single_guide_rna_design) | enabling | 72% | primary_source | Guide RNA design works with PAM compatibility to define targetable sites, but PAM recognition is the Cas9-side DNA constraint. |
|
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