Single-Guide RNA Design
Design of synthetic Cas9-compatible single-guide RNAs that combine targeting and scaffold features to direct Cas9 to intended genomic targets.
Core metadata
- ID: single_guide_rna_design
- Era: Modern
- First known date: 2012 (exact)
- Region: Global molecular biology research
- Review status: source_checked
- Maturity: established
Prerequisites
- Bioinformatics (bioinformatics)
- Programmable Cas9 Nuclease (cas9_programmable_nuclease)
- DNA Synthesis & Oligonucleotides (dna_synthesis_oligonucleotides)
Dependents
- Cas12 and Cas13 Editing Platforms (cas12_cas13_editing_platforms)
- CRISPR-Cas9 Genome Editing (crispr_gene_editing)
- CRISPRi and CRISPRa (crispri_crispra)
- PAM Recognition Constraint (pam_recognition_constraint)
- Prime Editing (prime_editing)
Fields
Field lanes
- Genome Editing / CRISPR-Cas: Assays & Safety
Node sources
- A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity (Science / PubMed, 2012, primary_paper) • Supports: node, maturity
- GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases (Nature Biotechnology, 2015, primary_paper) • Supports: node, maturity
Prerequisite edge evidence
Edge/source evidence summary:
- Prerequisite edges: 3
- Average edge confidence: 76%
- Prerequisite sources: 3
- expert_inference: 2
- primary_source: 1
| Prerequisite | Type | Confidence | Evidence level | Note | Sources |
|---|---|---|---|---|---|
| Programmable Cas9 Nuclease (cas9_programmable_nuclease) | required | 91% | primary_source | Cas9-compatible single-guide RNA design requires the Cas9 nuclease architecture because the sgRNA scaffold and targeting sequence are engineered to program Cas9 cleavage. |
|
| Bioinformatics (bioinformatics) | enabling | 68% | expert_inference | Bioinformatics provides a capability that enables this technology without being the only possible path. |
|
| DNA Synthesis & Oligonucleotides (dna_synthesis_oligonucleotides) | enabling | 68% | expert_inference | DNA Synthesis & Oligonucleotides provides a capability that enables this technology without being the only possible path. |
|
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