Programmable Cas9 Nuclease
Cas9 configured as an RNA-guided nuclease that can be programmed to cut matching DNA sequences.
Core metadata
- ID: cas9_programmable_nuclease
- Era: Modern
- First known date: 2012 (exact)
- Region: Global molecular biology research
- Review status: source_checked
- Maturity: established
Prerequisites
- CRISPR Adaptive Immunity (crispr_adaptive_immunity)
- Molecular Biology (molecular_biology)
- Protein Engineering (protein_engineering)
Dependents
- Base Editing (base_editing)
- CRISPR-Cas9 Genome Editing (crispr_gene_editing)
- PAM Recognition Constraint (pam_recognition_constraint)
- Single-Guide RNA Design (single_guide_rna_design)
Fields
Field lanes
- Genome Editing / CRISPR-Cas: Editing Platforms
Node sources
- A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity (Science / PubMed, 2012, primary_paper) • Supports: node, maturity
- The Nobel Prize in Chemistry 2020 (Nobel Prize, 2020, museum) • Supports: node, maturity
Prerequisite edge evidence
Edge/source evidence summary:
- Prerequisite edges: 3
- Average edge confidence: 73%
- Prerequisite sources: 3
- expert_inference: 2
- primary_source: 1
| Prerequisite | Type | Confidence | Evidence level | Note | Sources |
|---|---|---|---|---|---|
| CRISPR Adaptive Immunity (crispr_adaptive_immunity) | historical_predecessor | 78% | expert_inference | Discovery of CRISPR-Cas immunity precedes engineering Cas9 as a programmable nuclease. |
|
| Protein Engineering (protein_engineering) | enabling | 74% | primary_source | Protein engineering supports Cas9 domain characterization and later optimization, but the 2012 programmable nuclease platform is primarily RNA-guided rather than dependent on engineered Cas9 variants. |
|
| Molecular Biology (molecular_biology) | enabling | 68% | expert_inference | Molecular Biology provides a capability that enables this technology without being the only possible path. |
|
This page is generated from canonical era JSON and is indexable by URL.