CRISPRi and CRISPRa
Catalytically inactive Cas systems fused to regulatory domains to repress or activate genes without cutting DNA.
Core metadata
- ID: crispri_crispra
- Era: Modern
- First known date: 2013 (exact)
- Region: Global functional-genomics research
- Review status: source_checked
- Maturity: established
Prerequisites
- CRISPR-Cas9 Genome Editing (crispr_gene_editing)
- RNA Interference (rna_interference)
- Single-Guide RNA Design (single_guide_rna_design)
Dependents
- None.
Fields
Field lanes
- Genome Editing / CRISPR-Cas: Editing Platforms
Node sources
- CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes (Cell / PubMed, 2013, official_agency) • Supports: node, maturity
Prerequisite edge evidence
Edge/source evidence summary:
- Prerequisite edges: 3
- Average edge confidence: 71%
- Prerequisite sources: 3
- expert_inference: 3
| Prerequisite | Type | Confidence | Evidence level | Note | Sources |
|---|---|---|---|---|---|
| CRISPR-Cas9 Genome Editing (crispr_gene_editing) | enabling | 78% | expert_inference | CRISPRi/a adapts CRISPR targeting into regulation rather than cleavage-only editing. |
|
| RNA Interference (rna_interference) | enabling | 68% | expert_inference | RNA Interference provides a capability that enables this technology without being the only possible path. |
|
| Single-Guide RNA Design (single_guide_rna_design) | enabling | 68% | expert_inference | Single-Guide RNA Design provides a capability that enables this technology without being the only possible path. |
|
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