RNA Interference
Sequence-specific gene silencing by small RNAs, establishing a practical route to programmable knockdown of gene expression.
Core metadata
- ID: rna_interference
- Era: Modern
- First known date: 1998 (exact)
- Region: United States / C. elegans research community
- Review status: source_checked
- Maturity: established
Prerequisites
Dependents
- Cas12 and Cas13 Editing Platforms (cas12_cas13_editing_platforms)
- CRISPRi and CRISPRa (crispri_crispra)
Fields
Field lanes
- Genome Editing / CRISPR-Cas: Foundations
Node sources
- Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans (Nature / PubMed, 1998, primary_paper) • Supports: node, maturity
Prerequisite edge evidence
Edge/source evidence summary:
- Prerequisite edges: 3
- Average edge confidence: 65%
- Prerequisite sources: 3
- expert_inference: 3
| Prerequisite | Type | Confidence | Evidence level | Note | Sources |
|---|---|---|---|---|---|
| Molecular Biology (molecular_biology) | enabling | 68% | expert_inference | RNA interference depended on molecular-biology methods for designing and assaying gene-specific double-stranded RNA effects. |
|
| DNA Sequencing (dna_sequencing) | enabling | 68% | expert_inference | Sequence-specific RNAi design relies on knowing target gene sequences, but sequencing is an enabling substrate rather than a hard dependency for the natural phenomenon. |
|
| Bioinformatics (bioinformatics) | accelerates | 58% | expert_inference | Bioinformatics accelerates target selection and off-target analysis for practical RNAi workflows, but it was not a prerequisite for the 1998 discovery. |
|
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