Prime Editing
Search-and-replace genome editing that uses a Cas nickase, reverse transcriptase, and prime editing guide RNA to install precise edits.
Core metadata
- ID: prime_editing
- Era: Modern
- First known date: 2019 (exact)
- Region: Global genome-editing research
- Review status: source_checked
- Maturity: emerging
Prerequisites
- Base Editing (base_editing)
- NHEJ and HDR DNA Repair Editing (dna_repair_nhej_hdr)
- Single-Guide RNA Design (single_guide_rna_design)
Dependents
Fields
Field lanes
- Genome Editing / CRISPR-Cas: Editing Platforms
Node sources
- Search-and-replace genome editing without double-strand breaks or donor DNA (Nature, 2019, primary_paper) • Supports: node, maturity
- Prime editing for precise and highly versatile genome manipulation (Nature Reviews Genetics, 2023, review) • Supports: node, maturity
Prerequisite edge evidence
Edge/source evidence summary:
- Prerequisite edges: 3
- Average edge confidence: 68%
- Prerequisite sources: 3
- expert_inference: 3
| Prerequisite | Type | Confidence | Evidence level | Note | Sources |
|---|---|---|---|---|---|
| Base Editing (base_editing) | enabling | 68% | expert_inference | Base Editing provides a capability that enables this technology without being the only possible path. |
|
| Single-Guide RNA Design (single_guide_rna_design) | enabling | 68% | expert_inference | Single-Guide RNA Design provides a capability that enables this technology without being the only possible path. |
|
| NHEJ and HDR DNA Repair Editing (dna_repair_nhej_hdr) | enabling | 68% | expert_inference | NHEJ and HDR DNA Repair Editing provides a capability that enables this technology without being the only possible path. |
|
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