CRISPR Off-Target Profiling
Genome-wide assays and computational methods for detecting unintended CRISPR nuclease activity and improving editing safety.
Core metadata
- ID: crispr_off_target_profiling
- Era: Modern
- First known date: 2013 (decade)
- Region: Global genome-editing research
- Review status: source_checked
- Maturity: established
Prerequisites
- Bioinformatics (bioinformatics)
- CRISPR-Cas9 Genome Editing (crispr_gene_editing)
- DNA Sequencing (dna_sequencing)
Dependents
- Casgevy / Exa-cel (casgevy_exa_cel)
- Clinical Prime Editing (clinical_prime_editing)
- Ex Vivo CRISPR Cell Therapy (ex_vivo_crispr_cell_therapy)
- In Vivo CRISPR Therapeutics (in_vivo_crispr_therapeutics)
Fields
Field lanes
- Genome Editing / CRISPR-Cas: Assays & Safety
Node sources
- GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases (Nature Biotechnology, 2015, primary_paper) • Supports: node, maturity
Prerequisite edge evidence
Edge/source evidence summary:
- Prerequisite edges: 3
- Average edge confidence: 71%
- Prerequisite sources: 3
- expert_inference: 3
| Prerequisite | Type | Confidence | Evidence level | Note | Sources |
|---|---|---|---|---|---|
| CRISPR-Cas9 Genome Editing (crispr_gene_editing) | enabling | 78% | expert_inference | Off-target profiling is an assay layer for safer CRISPR use, not a prerequisite for discovering editing. |
|
| DNA Sequencing (dna_sequencing) | enabling | 68% | expert_inference | DNA Sequencing provides a capability that enables this technology without being the only possible path. |
|
| Bioinformatics (bioinformatics) | enabling | 68% | expert_inference | Bioinformatics provides a capability that enables this technology without being the only possible path. |
|
This page is generated from canonical era JSON and is indexable by URL.