Pooled CRISPR Screens
Library-scale CRISPR perturbation experiments that link guide RNAs to phenotypes for gene-function discovery.
Core metadata
- ID: pooled_crispr_screens
- Era: Modern
- First known date: 2014 (exact)
- Region: Global functional-genomics research
- Review status: source_checked
- Maturity: established
Prerequisites
- Bioinformatics (bioinformatics)
- Cell Culture (cell_culture)
- CRISPR-Cas9 Genome Editing (crispr_gene_editing)
- DNA Sequencing (dna_sequencing)
Dependents
- None.
Fields
Field lanes
- Genome Editing / CRISPR-Cas: Assays & Safety
Node sources
- Am I ready for CRISPR? A user's guide to genetic screens (Nature Reviews Genetics, 2018, review) • Supports: node, maturity
Prerequisite edge evidence
Edge/source evidence summary:
- Prerequisite edges: 4
- Average edge confidence: 71%
- Prerequisite sources: 4
- expert_inference: 4
| Prerequisite | Type | Confidence | Evidence level | Note | Sources |
|---|---|---|---|---|---|
| CRISPR-Cas9 Genome Editing (crispr_gene_editing) | enabling | 78% | expert_inference | Pooled screens build on CRISPR editing plus guide libraries and sequencing. |
|
| Cell Culture (cell_culture) | enabling | 68% | expert_inference | Cell Culture provides a capability that enables this technology without being the only possible path. |
|
| DNA Sequencing (dna_sequencing) | enabling | 68% | expert_inference | DNA Sequencing provides a capability that enables this technology without being the only possible path. |
|
| Bioinformatics (bioinformatics) | enabling | 68% | expert_inference | Bioinformatics provides a capability that enables this technology without being the only possible path. |
|
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