Zinc-Finger Nucleases
Engineered DNA-binding zinc-finger proteins fused to nucleases for programmable genome cleavage before CRISPR became dominant.
Core metadata
- ID: zinc_finger_nucleases
- Era: Modern
- First known date: 1996 (exact)
- Region: United States
- Review status: source_checked
- Maturity: established
Prerequisites
- DNA Sequencing (dna_sequencing)
- Protein Engineering (protein_engineering)
- Recombinant DNA & Genetic Engineering (recombinant_dna_genetic_engineering)
Dependents
Fields
Field lanes
- Genome Editing / CRISPR-Cas: Editing Platforms
Node sources
- Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain (Proceedings of the National Academy of Sciences / PubMed Central, 1996, primary_paper) • Supports: node, maturity
Prerequisite edge evidence
Edge/source evidence summary:
- Prerequisite edges: 3
- Average edge confidence: 83%
- Prerequisite sources: 3
- expert_inference: 1
- primary_source: 2
| Prerequisite | Type | Confidence | Evidence level | Note | Sources |
|---|---|---|---|---|---|
| Protein Engineering (protein_engineering) | required | 92% | primary_source | Zinc-finger nucleases require engineered protein-domain architecture: zinc-finger DNA-binding domains are fused to FokI cleavage domains to create sequence-directed nucleases. |
|
| Recombinant DNA & Genetic Engineering (recombinant_dna_genetic_engineering) | required | 90% | primary_source | ZFN construction requires recombinant-DNA cloning and expression methods: selected zinc-finger coding sequences are joined to FokI cleavage-domain coding sequence in cloning vectors before expression and testing. |
|
| DNA Sequencing (dna_sequencing) | enabling | 68% | expert_inference | DNA Sequencing provides a capability that enables this technology without being the only possible path. |
|
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