TALENs
Engineered transcription activator-like effector DNA-binding proteins fused to FokI nuclease domains for targeted genome cleavage.
Core metadata
- ID: talens
- Era: Modern
- First known date: 2011 (exact)
- Region: Global genome-editing research
- Review status: source_checked
- Maturity: established
Prerequisites
- DNA Sequencing (dna_sequencing)
- Protein Engineering (protein_engineering)
- Zinc-Finger Nucleases (zinc_finger_nucleases)
Dependents
- None.
Fields
Field lanes
- Genome Editing / CRISPR-Cas: Editing Platforms
Node sources
- Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting (Nucleic Acids Research / PubMed, 2011, primary_paper) • Supports: node, maturity
Prerequisite edge evidence
Edge/source evidence summary:
- Prerequisite edges: 3
- Average edge confidence: 76%
- Prerequisite sources: 3
- expert_inference: 2
- primary_source: 1
| Prerequisite | Type | Confidence | Evidence level | Note | Sources |
|---|---|---|---|---|---|
| Protein Engineering (protein_engineering) | required | 92% | primary_source | TALENs require engineered protein architecture: customized TALE repeat arrays provide DNA recognition and are fused to FokI nuclease domains for cleavage. |
|
| Zinc-Finger Nucleases (zinc_finger_nucleases) | enabling | 68% | expert_inference | Zinc-Finger Nucleases provides a capability that enables this technology without being the only possible path. |
|
| DNA Sequencing (dna_sequencing) | enabling | 68% | expert_inference | DNA Sequencing provides a capability that enables this technology without being the only possible path. |
|
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